Figure 2.

Aurora‐A stabilizes FOXM1 protein during cell cycle in late M phase and early G1 phase. A, Western blot analysis with indicated antibodies in control (si‐C) and Aurora‐A knock‐down (si‐A) MDA‐MB‐231 cells. B, Western blots analysis of the FOXM1 stability in MDA‐MB‐231 cells expressing either control siRNA or siRNA targeting Aurora‐A. Cells were treated with cycloheximide (CHX). Lysates were prepared at the indicated times after addition of CHX and probed with the indicated antibodies. C, Stabilization of FOXM1 by expression of Aurora‐A. Cells were transfected with control vectors and Aurora‐A overexpression vectors as indicated. D, Cells were transfected with si‐control and si‐Aurora‐A small RNA. Subsequently, MG‐132 was added to cells at last 6 h and lysates were analysed by Western blot. E, Cells were arrested at the G1‐S boundary by double thymidine block, released into fresh medium, and harvested at the indicated times. Cell lysates were subjected to Western blot assays with indicated antibodies. F, Cells were released from nocodazole block (prometaphase) for the indicated times. Cell extracts were subjected to Western blotting with indicated antibody. G, Cells were transfected with si‐control and si‐Aurora‐A small RNA, and real‐time RT‐PCR was performed to evaluate the M phase cell cycle genes