Figure 5.

Scratch test and Transwell assay and immunofluorescence show that silencing of lncRNA H19, overexpression of CDH1 or inhibition of CDH1 methylation suppresses migration and invasion and EMT of A549 cells. A and B, migration ability of A549 cells in each group after transfection detected by scratch test; C and D, invasion ability of A549 cells in each group after transfection measured by Transwell assay; E, protein bands and statistical plot of expression of EMT‐related genes determined by Western blot analysis; F, expression of EMT‐related genes measured by immunofluorescence assay. lncRNA H19, long non‐coding RNA H19; CDH1, E‐cadherin; NC, negative control; DMSO, dimethylsulfoxide; EMT, epithelial‐mesenchymal transition; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. *P < .05 vs the oe‐NC group; ^# P < .05 vs the DMSO group; ^& P < .05 vs the si‐NC group. The measurement data are presented as mean ± standard deviation, analysed by one‐way ANOVA. n = 20. The experiment was independently repeated three times