Figure 6.

RNA‐FISH and CHIP reveal that silencing of lncRNA H19 inhibits methylation of CDH1. A, expression of H19 in lung adenocarcinoma tissues and normal tissues retrieved from the TCGA database; B, subcellular localization of lncRNA H19 detected by RNA‐FISH. C, CpG island in the CDH1 promoter region based on the Meth Primer software; D, the binding site between lncRNA H19 and CDH1 promoter predicted by biological website; E, the luciferase intensity of the CDH1 wild‐type promoter region detected by luciferase reporter gene; F, the luciferase intensity of the M‐CDH1 determined by luciferase reporter gene; G, statistical chart of CHIP. lncRNA H19, long non‐coding RNA H19; CDH1, E‐cadherin; NC, negative control; DMSO, dimethylsulfoxide; RNA‐FISH, RNA fluorescence in situ hybridization; ChIP, chromatin immunoprecipitation; M‐CDH1, methylated CDH1; DNMT1, DNA methyltransferase 1; DNMT3A, DNA methyltransferase 3A; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. *P < .05 vs the NC group; ^# P < .05 vs the IgG group. The measurement data are presented as mean ± standard deviation, analysed by Student's t test. n = 3. The experiment was independently repeated three times