Figure 5.

Free miR‐499, not exosome‐associated miR‐499, released from injured NRCMs accounts for increased level of miR‐499 in RPMEC cells. A, Exosomes of NRCMs are 110 ± 27 nm in diameter by NanoSight NS300. B, Exosomes are positive for Alix, CD9 and Tsg101 and negative for Grp94 and calnexin. C, The CdM from NRCMs subjected to HX/R significantly increased miR‐499 in RPMEC cells compared to the that from NX control, and depletion of exosomes from CdM‐HX/R decreased miR‐499 expression by 25% (n = 3 and P < 0.05 for all). The expression of miR‐499 was normalized to that of U6 RNA. Experiments were performed three times in triplicate. Data are presented as ‘Mean ± SD’. Two groups comparison was performed using paired or non‐paired Student's t test, with * denoting P < 0.05