Figure 6.

SNHG6 induced EMT by binding UPF1 and activating the TGF‐β/Smad pathway. (A) qRT‐PCR analysis of Smad7 mRNA expression following UPF1 silencing **P < 0.01. (B) Western blot analysis of Smad7 protein expression following UPF1 silencing. (C) Western blot analysis of Smad7 protein expression following UPF1 up‐regulation. (D) Pearson's correlation analysis of the relationship between UPF1 and Smad7 expression levels in HCC tissues. (E) The expression of total and phosphorylation of key molecules of the TGF‐β pathways (Smad2, Smad3) were detected using Western blot. (F) The EMT markers were detected in HepG2 and Hep3B cells after cotransfection with UPF1 siRNA and control (scramble) or SNAI3‐AS1 shRNA