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. 2019 Jul 2;23(9):6072–6084. doi: 10.1111/jcmm.14470

Figure 2.

Figure 2

Generation of Hsd3b‐GFP‐positive cells after infection with sgRNA lentiviral vectors. A, Schematic diagram of the experimental procedure. B, Representative microscopy images of the stable Hsd3b‐dCas9‐MPH‐HFF cell lines constructed as described in Figure 1A. Human foreskin fibroblasts were infected with the indicated lentivirus at an MOI of 20, and at day 10 after infection with dCas9‐vp64 and MPH lentivirus, samples were stained with Hoechst 33342 (blue). Scale bars = 100 μm. C, Representative microscopy images showing the cellular appearance at day 4 after sgRNA transduction (MOI = 20). Scale bars = 100 μm (upper panel) or 50 μm (lower panel)