Figure 6.

In vitro, the CRISPR/dCas9 system leads to rapid epigenetic remodelling at target loci. A, C, E, Schematic representation of the target genomic loci. The sgRNA target sites upstream of the transcription start site (red bars) and the ChIP‐qPCR regions are indicated (black bars). B, D, F, Activation of the endogenous targets Nr5a1, Gata4 and Dmrt1 in human foreskin fibroblasts resulted in significant enrichment of H3K4me3 and H3K27ac at multiple loci at day 4 after infection (**P < 0.01, ***P < 0.001 n = 3 biological replicates). All fold enrichments are relative to IgG (negative control) and normalized to GAPDH