Fig. 4.

Comparison of 5–9 regime of intermittent fasting vs matched continuous CR in type 1 diabetic mice. (a) Experimental scheme with the STZ-treated mice (n = 5 for each group). STZ (40 mg/kg) was administered intraperitoneally for five consecutive days. An equal volume of citrate buffer was injected into control mice. (b) Body weight. The area under the curve is shown on the right. (c) Blood glucose levels. Blood samples were collected on the last day of each cycle. Mice were fasted for 6 h (morning fasting) before glucose measurements. The area under the curve is shown on the right. (d, e) Glucose tolerance test and insulin tolerance test at week 13. The area under the curve is shown on the right. (f) Serum insulin levels under fed state. Blood samples were collected after mice were sacrificed. (g, h) Levels of total triglycerides and total cholesterol in the blood. (i, j) Blood ALT and AST levels of the mice. Data are expressed as the mean ± SEM, n = 5 per group. Significant differences were assessed by one-way ANOVA followed by the Student-Newman-Keuls test where appropriate