Fig. 6.

Immunofluorescence analysis of the pancreatic islets of the mice. (a) Representative immunofluorescence staining of pancreatic sections of normal C57BL/6 mice, db/db mice and STZ-treated mice with or without diet intervention (the whole data are shown in Additional file 1: Figures S3, S4 and S5). The pancreatic sections were stained with antibodies against insulin (green color) and glucagon (red color). The nucleus was stained with Hoechst 33342 (blue color). Scale bar, 50 μm. (b, c, d, e) Quantitation of β cells and α cells per islet based on the immunofluorescence staining data