Fig. 4.

IncSNHG15 is associated with GBM malignancy and drug resistance. a Real-time PCR (a) and western blot (b) analyses of TMZ-R cells with lncSNHG15 silenced (KD) or overexpressed (OE). Expression level of lncSNHG15 is associated with oncogenic markers, CDK6, Sox2, β-catentin and EGFR as demonstrated by overexpressing lncSNHG15 (OE) and reduced expression of these markers was found in the lncSNHG15-knocked down (KD) TMZ-R cells. LncSNGH15-silenced TMZ-R cells appeared to form a significantly lower number of colonies (c) and nerospheres (d) as compared to NC and OE counterparts. **P < 0.01; ***P < 0.005. e Drug sensitivity assay. LncSNHG15-silenced TMZ-R cells became more sensitive (IC50, 225 μM) towards TMZ treatment while lncSNHG15-overexpresed TMZ-R became even more resistant (IC50 > 500 μM) against TMZ, as compared to NC control (IC50, 310 μM)