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. 2019 Aug 29;19:854. doi: 10.1186/s12885-019-6038-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Composition and structure of ALI multilayered co-cultures. Schematics of the in vitro model developed and representative LSCM images of the culture showing the F-actin (in red) and cell nuclei (in blue) organization in these cultures. The Z-stack LSCM images, clearly demonstrating the 3D architecture of the models developed, were reconstructed with ImageJ software to obtain the side view shown. Scale bars: 20 μm (objective lens, 63×)