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. 2019 Aug 28;22(1):61–68. doi: 10.2478/bjmg-2019-0011

Table 1.

The sequence of primers used in two stages nested-PCR.

Stage Primers Sequences Temperature
1 EBNA-1-1 5'-TAG TCA GTC ATC ATC ATC CG-3' 55 °C
EBNA-1-2 5'-GGG ATT TAT TCT TTA GTG CG-3'

2 EBNA-1-3 5'-GCC ATT TTT CCA CCC TGT AG-3'
EBNA-1-4 5'-ATT GAG GGC GTC TCC TAA CA-3' 60 °C