. 2019 Aug 28;19:753. doi: 10.1186/s12879-019-4349-9

Table 1.

Samples for technical validation of RLEP qPCR and 16S rRNA RT qPCR

Purpose	Sample type (No.)	Obtained from	Origin
“Must detect RLEP/16S rRNA (DNA)” samples	Nasal swab sample (4)^a	2 sequencing confirmed MB leprosy patients	Togo
“Must detect RLEP/16S rRNA (DNA)” samples	PCR standard (1 per RLEP and 16S rRNA qPCR)	Cloned RLEP/16S rRNA plasmids with known copy numbers	GenExpress, Berlin, Germany
“Must not detect RLEP/16S rRNA (DNA)” samples	Nasal swab sample (14)^a	7 endemic controls (healthy individuals)	Togo
	Nasal swab sample (10)^a	5 occupational contacts to untreated (MB) leprosy patients	Munich, DITM^b medical staff
	Nasal swab sample (6)^a	3 non-exposed healthy controls	Munich, DITM^b laboratory staff
	Swab sample (5)^a	5 PCR confirmed Buruli ulcer patients	Ghana [24]
	Fine needle aspirate (12)^a	11 PCR confirmed Buruli ulcer patients	Ghana [24]
	Swab sample (1)^a	3 Patients with PCR confirmed cutaneaous leishmaniasis	Munich, accredited diagnostic laboratories of DITM^b
	Punch biopsy sample (2)^a	3 Patients with PCR confirmed cutaneaous leishmaniasis	Munich, accredited diagnostic laboratories of DITM^b
	Mycobacterial culture (13)^a	M. abscessus, M. africanum, M. avium, M. bovis, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. malmoense, M. marinum, M. microti, M. tuberculosis, M. xenopi	National Reference Center for Mycobacteria, Borstel, Germany
	Bacterial culture (5)^a	Microbial flora colonizing human skin or nasal mucosa: Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes and Escherichia coli	Max von Pettenkofer-Institute, Ludwig-Maximilians-University, Munich, Germany

^a DNA extracts (additional file – protocol 1)

^b DITM, Department of Infectious Diseases and Tropical Medicine (accredited according to DIN EN ISO 15189)