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. 2019 Aug 28;19:753. doi: 10.1186/s12879-019-4349-9

Table 3.

Controls for RLEP qPCR and 16S rRNA RT qPCR assays

Control Purpose Material/ method
RLEP qPCR 16S rRNA RT qPCR
Negative extraction control To exclude contamination during extraction procedure Transport buffer extracted in the same way as samples NAa
Positive run control To ensure adequate performance of qPCR Cloned RLEP plasmid standard Cloned 16S rRNA plasmid standard
Negative no template control To exclude contamination during PCR set up DEPCa treated water DEPCb treated water
Internal positive control To exclude false negative results due to inhibition TaqMan exogenous internal positive control (IPC)c TaqMan exogenous internal positive control (IPC)c

aNA Not applicable

bDEPC Diethylpyrocarbonate

cApplied Biosystems, Frankfurt, Germany