Table 3.
Control | Purpose | Material/ method | |
---|---|---|---|
RLEP qPCR | 16S rRNA RT qPCR | ||
Negative extraction control | To exclude contamination during extraction procedure | Transport buffer extracted in the same way as samples | NAa |
Positive run control | To ensure adequate performance of qPCR | Cloned RLEP plasmid standard | Cloned 16S rRNA plasmid standard |
Negative no template control | To exclude contamination during PCR set up | DEPCa treated water | DEPCb treated water |
Internal positive control | To exclude false negative results due to inhibition | TaqMan exogenous internal positive control (IPC)c | TaqMan exogenous internal positive control (IPC)c |
aNA Not applicable
bDEPC Diethylpyrocarbonate
cApplied Biosystems, Frankfurt, Germany