Fig. 4.

WNT5B is a specific marker of BLBC with poor prognosis. a mRNA expression of WNT ligands and their receptors Frizzleds between luminal and BLBC cell lines are shown in the heatmap (data are extracted from CCLE and displayed by using GraphPad Prism 7 software). b mRNA and protein expression levels of WNT3A, WNT5A, and WNT5B in two normal breast cell lines, eight luminal, and seven BLBC cell lines by semi-q RT-PCR and western blot (S: Short exposure; L: Long exposure). c Expression of Wnt5a and Wnt5b in representative Her-2 positive, luminal A, luminal B and BLBC tissues by IHC (Red scale bar = 200 μm; Purple scale bar = 40 μm). d The association of Wnt5a or Wnt5b expression between basal-like and non-BLBC tissue subtypes were assessed using Pearson’s χ² test (***p = 0.0003, ****p < 0.0001). Prognostic value of WNT5A (e) and WNT5B (f) mRNA levels in human breast cancer, data obtained from the KM-plotter