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. 2019 Aug 22;10:1085. doi: 10.3389/fphys.2019.01085

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Copyright © 2019 Ma, Sahar, Jeong and Huh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Irisin gene expression and secretion during 3T3-L1 adipogenesis. (A,B) Gene expressions of PPARγ and FNDC5 were examined by real-time PCR during day D–2 to D+8 of adipogenesis. Quantifications were normalized to 18S in each reaction. ^∗P < 0.05 compared with D–2, †P < 0.05 compared with D–0. (C) Irisin secretion in media during adipogenesis was analyzed by ELISA. ^∗P < 0.05 compared with D–0.