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. 2019 Aug 22;10:1085. doi: 10.3389/fphys.2019.01085

FIGURE 2.

FIGURE 2

Effect of recombinant irisin and FNDC5 overexpression on adipogenesis. (A–C) 3T3-L1 cells were treated with or without 100 ng/ml irisin from D–2 to D+6 of adipogenesis. Gene expressions of transcription factors were measured by real-time PCR and normalized to 18S rRNA. (D–J) 3T3-L1 preadipocytes were transfected with control or plasmid expressing FNDC5. Two days confluent cells were induced to differentiate and were harvested at day + 5 and day + 8. (D–F) Gene expressions of PPARγ, CEBPα, and FABP4 were analyzed by real-time PCR and normalized to 18S. (G,H) Western blot analysis of transcription factors. (I,J) Representative pictures and quantification of Oil red O staining in FNDC5-overexpressed cells at day 8. ∗P < 0.05 compared with control at each time-point.