FIGURE 2.

Bacterial challenge test of phage Sfin-1 on different clinical isolates of Shigella spp. Clinically isolated species of (A) Shigella flexneri 2a, (B) Shigella dysenteriae 1, and (C) Shigella sonnei were grown (OD600 = 0.3) in 20 mL LB in presence of several antibiotics as described in “Materials and Methods” section, hervested by centrifugation, resuspended in 1 mL LB medium and infected with Sfin-1 at an MOI of 0.1, 0.01, and 0.001. After adsorption, the cultures were diluted 21 fold in LB and incubated for 5 h with shaking at 37°C. At different time intervals, viability of Shigella spp. was determined by spread plate method. As negative control Shigell spp. were grown in absence of Sfin-1 in presence of antibiotics. Two way ANOVA indicated significant difference between control and Sfin-1 infected sets (P < 0.0001, n = 3).