FIGURE 9.

Sfin-1 infections on proteinase K and periodate treated host. The effect of proteinase K and sodium periodate on adsorption of phage Sfin-1. Shigella flexneri 2a, Shigella dysenteriae 1, and Shigella sonnei cultures (OD600 = 0.3) were treated with proteinase K (250 mg/mL) or sodium periodate (200 mM NaIO₄) followed by Sfin-1 (MOI 0.0001) infection. Upon centrifugation, the phage titer in supernatant was determined as described in “Materials and Methods” section. Cells suspended in LB, cells incubated at 55°C in LB and cells in acetate buffer were used as control. The results are shown as residual PFU percentages. The phage titer in the control supernatant was set to 100%. Mean ± SD of three independent experiments are indicated. To determine the significance of the differences between group means, unpaired t-tests were performed between the controls and the tests. ^∗Significance level, i.e., P < 0.05, “ns” indicates non-significant. (A–C) Results of S. flexneri 2a, S. dysentariae1, and S. sonnei, respectively.