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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Neurobiol Dis. 2019 Jun 22;130:104502. doi: 10.1016/j.nbd.2019.104502

Figure 5. IL-1β induces PGC-1α expression in astroglia and this is blocked by WIN.

Figure 5

Astroglia were treated with vehicle, IL-1β or WIN alone or with WIN and then IL-1β for one day and then nuclear and cytoplasmic-enriched lysates were analyzed by immunoblot for PGC1α and TFAM expression, respectively. (A) Image of immunoblot of PGC1a in nuclear-enriched lysates and image of the total protein transferred to the membrane. (B). Quantification of PGC-1α band densitometry normalized to total protein densitometry. (C) Image of immunoblot of TFAM in cytoplasmic-enriched lysates and image of the total protein transferred to the membrane. (D) Quantification of PGC-1α band densitometry normalized to total protein densitometry. All graphs plot mean ± SEM. All experiments were performed in three independent experiments using at least two astroglial lines with different genetic background. Data were analyzed by one-way ANOVA.