Astroglia were treated with vehicle, WIN, IL-1β, and WIN+IL-1β for one day and then supernatants were analyzed by Mesoscale Discovery electrochemiluminescent assay. (A-F) Levels of cytokines in the supernatant: IL-6 (A), TNF-α (B), IL-12p70 (C), IFNγ (D), IL-4 (E) and IL-10 (F). All graphs plot mean ± SEM. All experiments were performed in three independent experiments using at least two astroglial lines with different genetic background. Data were analyzed by one-way ANOVA.