Table 2.
T and NK cell concentrations and subset distribution 28–365 days after transplantation.
| Cell subset concentration, 106/L, median (IQR) | Day 28 N = 106 | Day 56 N = 104 | Day 91 N = 99 | Day 180 N = 86 | Day 365 N = 66 |
|---|---|---|---|---|---|
| T cells (CD3) | 420 (225–553) | 425 (260–738) | 460 (240–750) | 835 (458–1400) | 1150 (680–2000) |
| CD4 | 190 (110–300) | 190 (113–280) | 210 (100–300) | 280 (170–420) | 395 (250–490) |
| CD8 | 150 (71–240) | 175 (100–395) | 230 (100–440) | 440 (198–950) | 650 (340–1425) |
| TCR γδ | 18 (6.5–44) | 21 (7.2–50) | 21 (6.6–53) | 36 (12–87) | 62 (19–111) |
| Vδ1 | 1.5 (0.7–3.4) | 1.6 (0.6–4.6) | 1.6 (0.6–6.8) | 4.4 (1.6–24) | 7.9 (3.3–35) |
| Vδ2 | 13 (4.8–42) | 17 (5.1–39) | 14 (4.0–38) | 16 (6.1–53) | 24 (7.2–62) |
| NonVδ1-nonVδ2 | 0.4 (0.2–0.8) | 0.3 (0.1–1.0) | 0.5 (0.2–1.3) | 0.8 (0.3–4.4) | 1.2 (0.5–7.3) |
| NK cells (CD16/56) | 280 (140–403) | 190 (110–330) | 160 (100–270) | 240 (130–388) | 235 (150–360) |
| CD16bright | 26 (14–60) | 25 (12–51) | 24 (10–47) | 25 (13–55) | 27 (15–56) |
| CD16/56 | 158 (82–258) | 113 (69–202) | 106 (65–184) | 152 (97–282) | 179 (112–294) |
| CD56bright | 57 (27–96) | 31 (17–63) | 23 (14–38) | 17 (12–32) | 17 (10–27) |
| Relative concentrations, % | |||||
| CD4/CD3 | 56 (44–65) | 49 (28–61) | 46 (25–57) | 37 (22–50) | 35 (23–48) |
| CD8/CD3 | 37 (31–52) | 45 (36–65) | 51 (34–68) | 59 (46–74) | 62 (47–75) |
| CD4/CD8, ratio | 1.5 (0.9–2.1) | 1.0 (0.4–1.8) | 0.9 (0.4–1.5) | 0.6 (0.3–1.1) | 0.6 (0.3–1.0) |
| Naïve CD4/CD4 | 28 (15–37) | 26 (12–37) | 26 (15–35) | 21 (12–32) | 22 (13–29) |
| Central memory CD4/CD4 | 40 (34–49) | 41 (33–49) | 40 (34–48) | 40 (31–48) | 43 (34–48) |
| Effector memory CD4/CD4 | 25 (19–37) | 29 (18–42) | 27 (19–39) | 32 (22–46) | 31 (23–42) |
| TEMRA CD4/CD4 | 0.8 (0.4–1.8) | 1.1 (0.6–2.5) | 1.3 (0.6–2.5) | 1.5 (0.9–4.8) | 1.7 (0.9–4.7) |
| HLA-DR CD4/CD4 | 21 (13–40) | 27 (17–51) | 31 (21–49) | 37 (24–51) | 26 (20–37) |
| Naïve CD8/CD8 | 23 (7.6–37) | 13 (4.4–33) | 13 (5.8–26) | 12 (5.9–22) | 9.4 (4.4–19) |
| Central memory CD8/CD8 | 4.7 (2.9–7.9) | 4.3 (2.3–8.4) | 5.2 (2.9–9.2) | 5.6 (3.1–9.5) | 5.6 (2.6–9.2) |
| Effector memory CD8/CD8 | 39 (27–56) | 40 (29–52) | 36 (28–47) | 32 (24–43) | 37 (29–52) |
| TEMRA CD8/CD8 | 21 (13–38) | 32 (18–45) | 36 (24–52) | 44 (33–57) | 39 (23–51) |
| HLA-DR CD8/CD8 | 40 (22–61) | 52 (26–75) | 58 (40–74) | 64 (50–78) | 52 (35–62) |
| Naïve TCR γδ/TCR γδ | 1.8 (0.9–4.0) | 1.6 (0.6–3.4) | 2.1 (0.8–4.3) | 2.0 (1.0–4.5) | 2.1 (0.7–4.5) |
| Central memory TCR γδ/TCR γδ | 1.2 (0.4–2.3) | 0.9 (0.3–2.6) | 0.9 (0.2–2.2) | 1.2 (0.3–3.5) | 2.1 (0.6–4.0) |
| Effector memory TCR γδ/TCR γδ | 51 (37–67) | 48 (32–61) | 38 (23–54) | 37 (22–52) | 43 (27–65) |
| TEMRA TCR γδ/TCR γδ | 46 (29–61) | 49 (35–65) | 52 (40–69) | 58 (38–72) | 47 (25–63) |
| HLA-DR TCR γδ/TCR γδ | 44 (28–58) | 40 (22–57) | 45 (30–61) | 47 (33–68) | 36 (25–53) |
| TCR γδ/CD3 T | 4.6 (2.6–11) | 4.5 (2.3–9.8) | 4.1 (2.0–10) | 3.7 (2.4–7.4) | 4.0 (2.1–7.6) |
| Vδ1/TCR γδ | 10 (3.3–24) | 11 (3.7–22) | 13 (3.8–30) | 28 (8.5–48) | 35 (10–56) |
| Vδ2/TCR γδ | 88 (70–95) | 86 (68–95) | 82 (60–95) | 67 (38–90) | 61 (31–86) |
| NonVδ1-nonVδ2/TCR γδ | 2 (0.8–5.8) | 2.1 (0.6–5.2) | 3.0 (0.9–7.8) | 3.9 (0.9–9.6) | 4.0 (1.1–12) |
| CD16bright/NK | 11 (6.2–24) | 14 (7.7–21) | 15 (8–22) | 12 (7.8–21) | 13 (7.3–19) |
| CD16CD56/NK | 59 (47–70) | 68 (54–77) | 68 (55–81) | 76 (65–83) | 80 (71–86) |
| CD56bright/NK | 23 (16–33) | 15 (10–24) | 13 (8.3–22) | 8.7 (5.1–14) | 7.4 (3.7–13) |