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C9–PNA–FITC cellular uptake is predominantly in the cytoplasm. Confocal microscopy images of U87MG glioblastoma cells incubated with C9–PNA–FITC or dK4–PNA–FITC (at 0.5 μM) after an incubation of 3 h at 37 °C. (A) PNA–FITC alone (green); (B) overlay of PNA–FITC (green) with Lyso-Tracker Red (red) staining of lysosome (C); overlay of PNA–FITC (green) with Hoechst (blue) staining of nuclei; and (D) overlay of both stains with PNA–FITC.
