Table 1. PNA Conjugates as antimiRs (antimiR-155) with/without FITC Labeling and Their Corresponding ESI Mass Assignmentsa.
name | description | construct (N′ to C′) | mass (daltons) (calc) | mass (daltons)(found) |
---|---|---|---|---|
dK4–PNA | PNA conjugated to dK4 | 5′-ACCCCTATCACAATTAGCATTAA-3′-dK4 | 6671.70 | 6674.20 |
dK4–PNA-Scr | scrambled PNA conjugated to dK4 | 5′-ACCCAATCGTCAGATTCCATATA-3′-dK4 | 6671.70 | 6676.70 |
dK4–PNA–FITC | PNA conjugated to dK4 and FITC | FITC-Ahx-5′-ACCCCTATCACAATTAGCATTAA-3′-dK4 | 7174.24 | 7177.56 |
C9–PNA | PNA conjugated to C9 | 5′-ACCCCTATCACAATTAGCATTAA-3′-C9 | 7513.63 | 7516.5 |
C9–PNA-Scr | scrambled PNA conjugated to C9 | 5′-ACCCAATCGTCAGATTCCATATA-3′-C9 | 7513.63 | 7517.34 |
C9–PNA–FITC | PNA conjugated to C9 and FITC | FITC-Ahx-5′-ACCCCTATCACAATTAGCATTAA-3′-C9 | 8022.22 | 8018.76 |
C9 = cyclic-(F–f−Φ–R–r–R–r−γE)K and Ahx = 6-aminohexanoic acid