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Reference curves for quantitative determination of argatroban (A) and lepirudin (B) concentrations adapted to the BCS Analyzer. Linearity existed up to a concentration of 2.5 µg/mL. The measurement range of the ecarin chromogenic assay (ECA) assay in the study was 0.0 ng/mL to 1.8 µg/mL for argatroban (mean value: 0.41 ± 0.35) and 0 µg/mL to 1.0 ng/mL for lepirudin (mean value: 0.20 ± 0.21). Y-axis: ECA reaction time (seconds).
