FIG 10.

SHIV-specific CD4⁺ and CD8⁺ T cell responses during SHIV.CH505 infection. SHIV-specific responses against SIVmac239 Gag or HIV-1 Consensus C Env were characterized in PBMCs stimulated with overlapping peptide pools prior to and after SHIV infection by flow cytometry. Frequencies were considered positive after DMSO-stimulated and baseline subtractions. (A) Mean frequency of CD4⁺ or CD8⁺ T cells with cytokine (IFN-γ, IL-2, and TNF-α) or cytolytic effector (CD107a⁺/granzyme B [GrzB]⁺) functions. (B and C) Boolean gating was performed to identify the total frequency of CD4⁺ or CD8⁺ T cells with any of the effector functions described above. In panels B and C, each animal is represented by a different symbol. Statistical differences between week 8 and 16 p.i. time points were calculated using a multiple t test with corrections for multiple comparisons using the Holm-Sidak method.