FIG 3.

Minimal disruption of CD4⁺ T cell subsets during acute SHIV.CH505 infection. CD4⁺ T cell subsets were characterized in whole blood, rectum, colon, and lymph node tissue of SHIV.CH505-infected rhesus macaques by flow cytometry. (A) Percentage of CD3⁺ CD4⁺ T cells of CD45⁺ leukocytes in whole blood at all time points. (B) Absolute number of CD4⁺ T cells per μl of blood at all time points. (C) Percentage of CD3⁺ CD4⁺ T cells of CD45⁺ leukocytes in rectum, colon, and lymph node tissue at all time points. (D) Percentage of CD4⁺ central memory cells (CD28⁺ CD95⁺ CCR7⁺) of CD4⁺ T cells in rectum, colon, and lymph node at all time points. (E) Percentage of CD4⁺ effector memory cells (CD28⁻ CD95⁺ CCR7⁻) of CD4⁺ T cells in rectum, colon, and lymph node at all time points. (F) Percentage of CD4⁺ transitional memory cells (CD28⁺ CD95⁺ CCR7⁻) of CD4⁺ T cells in rectum, colon, and lymph node at all time points. In all panels, each animal is represented by a different symbol. Statistical significance between the post-SHIV infection time points and pre-SHIV infection baseline was calculated using Friedman’s test and Dunn’s multiple-comparison test.