TABLE 1.

Primers used for RT-PCR detection and RT-qPCR quantification of DENV-1, ZIKV, CFAV, and Aedes aegypti housekeeping genes rp49 and rps7

Genome	Genomic region	Application	Direction	Primer sequence (5′ → 3′)	Length of PCR product (bp)	Annealing temp (°C)
DENV-1	NS5	Detection	Forward	GGAAGGAGAAGGACTCCACA	105	60
DENV-1	NS5	Detection	Reverse	ATCCTTGTATCCCATCCGGCT	105	60
ZIKV	NS1	Detection	Forward	GTATGGAATGGAGATAAGGCCCA	191	60
ZIKV	NS2A	Detection	Reverse	ACCAGCACTGCCATTGATGTGC	191	60
CFAV	NS3	Quantification	Forward	ACACGAGTGAAGCTGGTTGA	92	56
CFAV	NS3	Quantification	Reverse	ACATACGTTCCTGGTTCCCG	92	56
CFAV	E	Detection	Forward	GCTTCAAGTGGGGGATTGGA	345	62
CFAV	E	Detection	Reverse	CAACTTTCTCCATGCCGTGC	345	62
CFAV	5′ UTRa	Genomic cDNA amplification	Forward	AGTTTATAAAAACTTCGGCTTGGCT	10,696	60
CFAV	3′ UTR	RT, genomic cDNA amplification	Reverse	AGCGCATCTATGGTATAGAAAAGA	10,696	60
Aedes aegypti	rp49	Housekeeping gene quantification	Forward	ACAAGCTTGCCCCCAACT	97	60
Aedes aegypti	rp49	Housekeeping gene quantification	Reverse	CCGTAACCGATGTTTGGC	97	60
Aedes aegypti	rps7	Housekeeping gene detection	Forward	GGGACAAATCGGCCAGGCTATC	292	64
Aedes aegypti	rps7	Housekeeping gene detection	Reverse	TCGTGGACGCTTCTGCTTGTTG	292	64

^aUTR, untranslated region.