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. 2019 Aug 28;93(18):e00539-19. doi: 10.1128/JVI.00539-19

FIG 2.

FIG 2

Activated STAT3 promotes the replication of RABV. (A) CVS-treated BHK-21 cells were treated with niclosamide for 24 h, and STAT3 (P/T) and CVS P protein levels were detected by Western blotting. (B) The relative expression levels of the above-described proteins were analyzed by the use of ImageJ software and normalized to the α-tubulin expression level. (C to J) CVS-treated BHK-21 cells were transfected with STAT3-specific siRNA (C to F) or the Flag-STAT3 plasmid (G to J) for 48 h. (C, G) The expression of SOCS3/STAT3 (P/T) and CVS P was detected by Western blotting. (D, H) The relative expression levels of the above-described proteins were analyzed by the use of ImageJ software and normalized to the β-actin expression level. (E, F, I, J) CVS P mRNA levels and viral titers were analyzed by, respectively, qRT-PCR and DFA. *, P < 0.05; **, P < 0.01. Each experiment was independently repeated at least three times. si-NC, negative-control siRNA.