FIG 3.

SOCS3 inhibits the replication of RABV. CVS-treated BHK-21 cells were transfected with Flag-SOCS3 plasmid (A to D) or SOCS3-specific siRNA (E to H) for 48 h. (A, E) The expression levels of SOCS3/STAT3 (P/T) and CVS P were detected by Western blotting. (B, F) The relative expression levels of the above-described proteins were analyzed by the use of ImageJ software and normalized to the β-actin expression level. (C, G) The CVS P mRNA levels were analyzed by qRT-PCR. (D, H) The viral titers of CVS-11 were detected by DFA. *, P < 0.05; **, P < 0.01. Each experiment was independently repeated at least three times.