Total KoRV and KoRV subtype expression by koala health outcome. Box and whisker plots are displayed for each category, and individual sample results are shown as black dots. Healthy koalas (N = 11) were sampled a total of 53 times, while koalas that developed cystitis (N = 5) were sampled a total of 19 times during this study. Using a mixed model ANOVA (to account for repeated measures) on the last 3 samples from each koala, total KoRV-pol expression levels (F[1,14] = 10.480, P = 0.006) and KoRV-D env expression levels (F[1,14] = 13.760, P = 0.002) were significantly different between healthy koalas and koalas that developed cystitis.