FIG 7.

Paramyxovirus V proteins trigger MAVS degradation. (A) HeLa cells were transfected with either empty vector or Flag-tagged V of NDV, NDV ΔDM2, measles virus, Sendia virus, Hendra virus, mumps, Nipah virus, or PIV5. At 12 hpt, cells were transfected with poly(I·C) (20 μg/ml). Cells were harvested at 12 hpt with poly(I·C) and detected using immunoblot analysis with anti-MAVS, anti-Flag, or anti-β-actin antibody. (B) The intensity band ratio of MAVS to β-actin. Representative results are shown with graphs representing the ratio of MAVS to β-actin normalized to the control condition. Data are presented as means from three independent experiments. (C) HeLa cells were cotransfected with p-125Luc, PRL-TK, and either empty vector or Flag-tagged V of NDV, NDV ΔDM2, measles virus, Sendai virus, Hendra virus, mumps virus, Nipah virus, or PIV5. At 12 hpt, cells were mock treated or transfected with poly(I·C) (20 μg/ml). Cells were harvested at 12 hpt and assessed for luciferase activity. The results are presented as relative luciferase activity. Data are presented as means from three independent experiments. #, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001.