FIG 1.

VSV and VSV-GP show different serum sensitivities if produced on different cell lines. VSV (A) and VSV-GP (B) were produced on different cell lines: murine L929, hamster BHK-21, African green monkey Vero, and human A549. Serum resistance assays with 50% NHS were performed with an incubation period of 45 min at 37°C. Plaque titrations were conducted to analyze the remaining titer. GMEM (w/o) and heat-inactivated NHS (hiNHS) served as controls. Mean values with standard deviations (SD) of independent experiments (N) are shown. Data were analyzed by GraphPad Prism software using ANOVA, followed by Dunnett’s multiple-comparison test (****, ***, and **, significant at P < 0.0001, P < 0.001, and P < 0.01, respectively).