FIG 5.

CML of VSV-GP can be reduced by inhibition of natural Abs using soluble α-Gal and Neu5Gc. (A) A virus capture assay using Abs against GP, α-Gal, or Neu5Gc reveals the presence of α-Gal on VSV-GP(L929) and Neu5Gc on VSV-GP(Vero). Sera were preincubated with soluble disaccharide Galα1,3Gal (α-Gal) or sialic acid N-glycolylneuraminic acid (Neu5Gc) prior to the addition of viral particles. N-Acetylneuraminic acid (Neu5Ac) served as a negative control. Serum sensitivity assays of VSV-GP(L929) (B) and VSV-GP(Vero) (C) were performed, and infectious viral titers were determined by plaque assays. The data show the results from two independent experiments with two technical replicates. Mean values are shown, and error bars indicate the SD.