FIG 6.

URMC-099 inhibits ZIKV-induced inflammatory cytokine production. (A to D) SNB-19 cells were inoculated with ZIKV (MOI = 0.1) for 12, 24, 36, or 48 h. The relative mRNA level of IL-6, IL-8, TNF-α, or MCP-1 was assayed by qRT-PCR. Uninfected SNB-19 cells in each time point were set as negative controls. (E to H) SNB-19 cells were inoculated with ZIKV (MOI = 0.1) in the absence or presence of URMC-099 (8, 40, or 200 nM) for 48 h. qRT-PCR was performed to measure IL-6, IL-8, TNF-α, or MCP-1 expression. Blank cells were used as negative controls. (I to L) Quantification of secreted IL-6 and IL-8 from SNB-19 cells by ELISA. Data are presented as the mean ± SEM. *, P < 0.05; **, P < 0.01.