FIG 1.

NSE-CD46⁺ mice survive MV challenge but fail to clear viral RNA. (A) NSE-CD46⁺ (n = 10) and NSE-CD46⁺ RAG2 KO (n = 8) mice were challenged i.c. with 1 × 10⁴ PFU of MV-Ed and monitored daily for survival. (B) Weights of all mice from panel A were obtained throughout the infection time course and compared to baseline. Percent weight gain or loss was then calculated. (C) NSE-CD46⁺ mice were challenged i.c. with 1 × 10⁴ PFU of MV-Ed and were sacrificed at the indicated day postinfection. RNA was purified from perfused brains and analyzed by RT-qPCR (using random hexamers to synthesize cDNA). (D) MV nucleoprotein mRNA in NSE-CD46⁺ mice challenged i.c. with MV-Ed was detected using the approach outlined above, substituting oligo(dT) primers to generate cDNA. Data are represented using the ΔΔC_T method. Results are representative of at least 3 independent experiments with 5 to 10 mice per group. (E) NSE-CD46⁺ (n = 6) mice were challenged i.c. with 1 × 10⁴ PFU of MV-Ed and were sacrificed at 2 years postinfection. RNA was purified from perfused brains and analyzed by RT-qPCR (using random hexamers to synthesize cDNA). Data are represented using the ΔΔC_T method. *, P < 0.05, Mann-Whitney U test.