FIG 5.

Sublethal irradiation leads to increased expression of MV protein. (A to D) Immunofluorescence of whole-brain tissue obtained from uninfected mice (A), immunocompetent persistently infected (134 days postinfection) mice (B), persistently infected mice sublethally irradiated for 7 days (C), and persistently infected mice sublethally irradiated for 14 days (D). Nuclear staining (Hoechst [blue]) and MV staining (human polyclonal MV antibody [red]) were used. Arrows indicate MV-positive cells. Scale bar = 50 μm. (E) NSE-CD46⁺ mice were challenged i.c. with 1 × 10⁴ PFU of MV-Ed for at least 90 dpi, followed by 14 days of sublethal irradiation. Shown is Western blot analysis of protein purified from whole-brain tissue of individual mice probed for MV nucleoprotein and GAPDH.