FIG 5.

CHIKV infection triggers replication and transcription of template RNAs produced from the CMV-Fluc-Gluc, HSPolI-Fluc-Gluc, Ubi-Fluc-Gluc, and AlbPolI-Fluc-Gluc plasmids. (A) U2OS cells were transfected with the CMV-Fluc-Gluc or HSPolI-Fluc-Gluc plasmid. At 18 h posttransfection, cells were either infected with CHIKV at an MOI of 10 or mock infected. Cells were collected at 24 hpi and lysed, and Fluc (replication, left) and Gluc (transcription, right) activities were measured and normalized to the number of transfected cells. (B) C6/36 cells were transfected with the Ubi-Fluc-Gluc or AlbPolI-Fluc-Gluc plasmid. At 36 h posttransfection, cells were either infected with CHIKV at an MOI of 10 or mock infected. Cells were collected at 72 hpi and lysed, and Gluc activities were measured and normalized to the number of transfected cells. Each column represents an average from at least three independent experiments; error bars represent standard deviations. ****, P < 0.0001 (Student’s unpaired t test). RLU, relative light units.