TABLE 1.
Sequences of promoter-CHIKV 5′ UTR junctions for plasmids expressing template RNAs
| Plasmid | Promoter residues −10 to −1 | Downstream part of promoter sequence | Leader sequence + HH RZa | CHIKV residues 1 to 10 |
|---|---|---|---|---|
| CMV-Fluc-Gluc | AGTGAACCGT | ATGGCTGCGT | ||
| CMV-HH-Fluc-Gluc | AGTGAACCGT | GTATTTTTACAACAATTACCAACAACAACAAACAACAAACAACATTAC AATTACTATTTACAATTACAATGGCATACACACAGACAGCTACCACAT CAGCTTTGCTGGACACTGTCCGAGGAAACAACTCCTTGGTCTAATAAT AAAGCCATCTGATGAGAGCGAAAGCTCGAAACTGGAGGAAACTCCAGTC |
ATGGCTGCGT | |
| HSPolI-HH-Fluc-Gluc | CCGGGTTATT | GCTGACACGCTGTCCTCTGG | AGCCATCTGATGAGAGCGAAAGCTCGAAACTGGAGGAAACTCCAGTC | ATGGCTGCGT |
| HSPolI-Fluc-Gluc | CCGGGTTATT | ATGGCTGCGT | ||
| CGPolI-Fluc-Gluc | TGACACGCTT | ATGGCTGCGT | ||
| Ubi-Fluc-Gluc | AAACCAGCTC | ATGGCTGCGT | ||
| AegPolI-HH-Fluc-Gluc | AAAACCCTTC | AGGGAGGAAGGCAGTGT GCGTGGACCGGCAGGAA AATGTTCCGAAAGCAA |
AGCCATCTGATGAGAGCGAAAGCTCGAAACTGGAGGAAACTCCAGTC | ATGGCTGCGT |
| AegPolI-Fluc-Gluc | AAAACCCTTC | ATGGCTGCGT | ||
| AlbPolI-Fluc-Gluc | AAAACCCTAT | ATGGCTGCGT |
a
The boldface sequence represents the HH RZ.