FIG 6.

RLORF4 deficiency facilitates CD8⁺ T cell response and attenuates viral replication. (A) Growth properties of wtGA and GAdRLORF4 in CEF cell cultures. (B to E) Chickens were infected with 2,000 PFU of wtGA or GAdRLORF4. (B and C) Virus genome copy numbers in spleen (B) and thymus (C) were monitored at the indicated time points. (D and E) Chicken peripheral blood lymphocytes were obtained to analyze the percentage of CD8⁺ T cells at the indicated time points postinoculation. Statistical analysis was performed with Student’s t test (*, P < 0.05, **, P < 0.01, ***, P < 0.001).