FIG 3.

CsA resistance analysis of engineered rEAV mutants reflecting the consensus sequences of CsA-1, -2, and -3. Huh7 cells in 96-well plates were infected (MOI, 0.05) with rEAV^wt (control; black line) or mutants reflecting the consensus sequences of CsA-1, -2, and -3. Cells were incubated for 3 days in the presence of 0 to 50 μM CsA, and cell viability was monitored using a commercial assay. The cytotoxicity of CsA treatment only was monitored in parallel in mock-infected Huh7 cells (light gray line). Above each panel, the EC₅₀ value of CsA inhibition is indicated (see also Table 1). Graphs show the results (average and SD) of a representative experiment that was performed in quadruplicate. All experiments were repeated at least twice.