FIG 5.

Adaptive mutations in EAV nsp5 do not affect the morphology of virus-induced double membrane vesicles. Huh7 cells were infected with rEAV^wt (B and E) or rEAV^QYA (C and F) at an MOI of 5 or were mock infected (A and D). After an incubation of 11 h (untreated [A to C]) or 14 h (CsA treated [D to F]), cells were fixed and processed for transmission electron microscopy. DMV formation is very similar upon infection with rEAV^wt and rEAV^QYA, while CsA treatment induces dilated ER both in mock-infected and in infected cells. Scale bars: 2 μm (left images) and 500 nm (middle and right images).