TABLE 2.
Mutations identified after serial passaging of EAV in the presence of increasing concentrations of CsA or ALV
| Virus | Mutation in: |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 5′ UTR | nsp1 | nsp2 | nsp5 |
nsp10 | GP2b | GP4/OR5a protein | |||
| CsA-treated replicates | |||||||||
| EAVwt P7 | |||||||||
| EAV CsA-1 P7 |
Q21R (mixa
) (CAA→CGA) |
Y113H (UAC→CAC) |
A134V (GCU→GUU) |
||||||
| EAV CsA-2 P7 | nt 43 (A→G) |
Y133H (UAC→CAC) |
Y387H (UAC→CAC) |
||||||
| EAV CsA-3 P7 |
A199T (GCU→ACU) |
L8S (UUG→UCG) |
Q21R (CAA→CGA) |
||||||
| ALV-treated replicates | |||||||||
| EAVwt P10 | |||||||||
| EAV ALV-1 P10 | nt 45 (A→G) |
H114R (CAC→CGC) |
Y113H (UAC→CAC) |
A134V (GCU→GUU) |
Y151C/I14V (TATC→TGTC)b |
||||
| EAV ALV-2 P10 | nt 47 (U→C) |
T39N (ACC→AAC) |
H114R (CAC→CGC) |
Q21R (CAA→CGA) |
M62T (AUG→ACG) |
H11L (CAT→CTT) |
|||
a
Two variants were detected: nsp5 Q21 (wild type) and R21 (mutated sequences).
b
The open reading frames for EAV GP4 and the ORF5a protein overlap.