FIG 5.

Binding activity of P[5] VP8* domains and the amount of Lewis^y, H, and A HBGAs in saliva. Binding of the GST-VP8* domains was tested on a panel of human (capital letter H on the x axis, indicated by yellow bars), bovine (capital letter B on the x axis, indicated by green bars), and porcine (capital letter P on the x axis, indicated by blue bars) saliva samples, using an anti-GST antibody (1:1,000 dilution), followed by the addition of an HRP-conjugated goat anti-mouse IgG antibody. (A to C) The binding results for individual saliva samples were sorted by their Le^y (A), H (B), and A (C) signals. No correlation was observed between the salivary Le^y, H, and A signals and VP8* binding levels in either P[5]-bearing strain. Binding of saliva samples was visualized using TMB, which was measured at 450 nm in three independent experiments. Error bars represent means ± the SD.