FIG 6.

Expression of the αGal epitope and infectivity of the bovine G6P[5] WC3 and bovine-human mono-reassortant G4P[5] strains in various cell lines. (A) The expression level of the αGal epitope in Old World monkey (MA104), bovine (MDBK), human (Caco-2), and porcine (LLC-PK) cells was determined using an immunofluorescence assay with MAbs specific to the αGal epitope. Scale bars, 20 μm. (B) The infectivity of both P[5]-bearing strains (MOI, 1 FFU/cell) in the cell lines was assessed using an immunofluorescence assay with MAbs specific to the RVA VP6 protein at 15 h postinfection. All experiments were performed on three independent occasions. Scale bars, 200 μm. Panels A and B show representative images.