TABLE 1.

Primers used for amplification of the RVA VP8*, VP7, and NV P domain coding regions

Strain	Sequence (5′–3′)a	Construct generated	Restriction enzyme site	Sense
G6P[1] NCDV	GTGGATCCATGGCTTCACTCATTTATAGAC	VP8* full-length	BamHI	Positive
	GCCTCGAGTCATCTCGTATTTTGTATTGGTGG		XhoI	Negative
G6P[5] WC3	GTGGATCCATGGCTTCGCTCATATACAG	VP8* full-length	BamHI	Positive
	GCCTCGAGTCATCTGGTGTTTTGGATTG		XhoI	Negative
	GGCTTTAAAAGAGAGAATTTCCGT	VP7 full-length		Positive
	GGTCACATCATACAATTCTAATCT			Negative
G4P[5] RotaTeq	GTGGATCCATGGCTTCGCTCATATACAG	VP8* full-length	BamHI	Positive
	GCCTCGAGTCATCTGGTGTTTTGGATTG		XhoI	Negative
	GGCTTTAAAAGAGAGAATTTCCGT	VP7 full-length		Positive
	GGTCACATCAAACAGTTCTATTTT			Negative
G3P[3] CU-1	GTGGATCCATGGCTTCGCTCATTTATAGAC	VP8* full-length	BamHI	Positive
	GCCTCGAGTCATCTTGTATTTTGAATCGGTGG		XhoI	Negative
G11P[25] Dahaka	GTGGATCCATGGCTTCGTTAATTTACAGAC	VP8* full-length	BamHI	Positive
	GCCTCGAGTCAACGTGTATTCTGAATTGGTGG		XhoI	Negative
VA387 (GII.4)	GCACGGATCCTCAAGAACTAAACCATTCACC	P-CDCRGDCFC	BamHI	Positive
	GCATGCGGCCGCTTAGCAAAAGCAATCGCCACGGCAATC GCA TAATGCACGTCTGCGCCCCGC		NotI	Negative

^aUnderlining indicates the restriction enzyme site described in column 4.