FIG 3.

Partial least-squares discriminant analysis (PLS-DA) score plot of metagenomic (A) and metatranscriptomic (B) gene abundance data sets of CDI⁺ and CDI⁻ individuals. Two-dimensional PLS-DA plots generated within MixOmics reveal distinct clustering of CDI⁺ (blue) and CDI⁻ (orange) samples using CPM-normalized counts of metagenome functional gene data and metatranscriptome expression data, indicating distinct functional gene and expression profiles between disease states. Complementary area under the receiver operating characteristic (AUROC) measures were calculated to validate the metagenome (AUROC of 0.934) and metatranscriptome (AUROC of 1.0) PLS-DA models. An improved ability for the model to differentiate between CDI⁺ and CDI⁻ samples was observed for the metatranscriptome data set.