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. 2019 Aug 29;14(8):e0221762. doi: 10.1371/journal.pone.0221762

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© 2019 Calabrese et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Surface pluripotency marker TRA-1-60 specific or isotype IgG life immunofluorescence analysis of Li-iPSC colonies derived from patients C101 and C496 (scale bar 250μm). (B) Pluripotency gene (SOX2 and OCT3/4) expression in PLCs and Li-iPSCs derived from patients C101 and C496 was quantified by RT-qPCR using total cellular RNA. Shown is the relative expression of the transcripts compared to GAPDH (mean +/- SD; ND = not detectable). (C) Hematoxylin and eosin staining of formalin fixed and paraffin embedded sections from teratomas isolated from NOD-SCID mice 50 days after subcutaneous injection of Li-iPSCs from patients C101 or C496 (scale bar 500μm).