Table 1. Antibodies employed for Immunohistochemistry.
| Antigen | Species | Company | Staining Method | Working Dilution |
|---|---|---|---|---|
|
NKI-beteb (Pmel17) |
mouse-anti-human | Monosan, Uden, Netherlands | IF (green) double with KIT (red) | 1:20 |
| c-KIT | rabbit-anti-human | Dako, Hamburg, Germany | IF (red) double with NKI-beteb (green) | 1:100 |
| TrkA | rabbit-anti-human | Santa Cruz Biotechnologie, Inc., Heidelberg, Germany | IF (red) double with NKI-beteb (green) | 1:50 |
| p16 | rabbit-anti-human | Santa Cruz Biotechnologie, Inc., Heidelberg, Germany | IF (red) double with NKI-beteb (green) | 1:50 |
For red label tetramethyl-rhodamine isothiocyanate-labeled, for green label Cy2-labeled goat anti-rabbit or goat anti-rat secondary antibodies (1:200; Jackson ImmunoResearch, West Grove, PA) were used. Secondary antibodies were added for 1 hour at 37°C in PBS with 2% normal goat serum. All sections were counterstained with 4-,6-diamidino-2-phenylindol-dihydrochlorid (DAPI; Boehringer Mannheim, Mannheim, Germany) for identification of cell nuclei. Abbreviations used: IF–immunofluorescence.