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. 2019 Aug 29;14(8):e0221878. doi: 10.1371/journal.pone.0221878

Fig 2. Staining efficiency and microcarrier attachment.

Fig 2

(A) Keratinocytes stained with 5 μM 5(6) carboxyfluorescein-N-hydroxysuccinimidyl ester (CFSE) after 24 hours in spinner flask culture. (B) (4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)-staining of CFSE-stained keratinocytes on microcarriers after 72 hours in spinner flask culture, indicating viable cells. (C) Melanocytes stained with 5 μM CFSE after 48 hours in spinner flask culture. Scale bar = 200 μm (D) MTT-staining of melanocytes on microcarriers after 72 hours in spinner flask culture, indicating viable cells. In both the CFSE and MTT stainings, cells occupying outer surface as well as the inner pores of the microcarriers can be observed. (E) Staining efficiency of 5 μM CFSE investigated 24 hours after staining; 93.3 ± 2.6% of keratinocytes and 93.0 ± 3.5% of melanocytes were positive for CFSE and nuclear staining (mean ± SD, n = 3). Ker = keratinocytes; Mel = melanocytes. Scale bars = 300 μm.